"These studies suggest that VEGF-A interacts with NRP-1 and GIPC1 to regulate α6/β4-integrin, FAK, Src, PI3K/PDK1, LATS1 signaling to increase YAP1/∆Np63α accumulation to drive ECS cell survival, angiogenesis, and tumor formation."
"A co-immunoprecipitation analysis revealed that VEGF-A induced interactions between NRP1 and GIPC1, a scaffold protein, and complex formation between GIPC1 and Syx, a RhoGEF."
"These studies suggest that VEGF-A interacts with NRP-1 and GIPC1 to regulate α6/β4-integrin, FAK, Src, PI3K/PDK1, LATS1 signaling to increase YAP1/ΔNp63α accumulation to drive ECS cell survival, angiogenesis, and tumor formation."
"VEGF-A binds the b1 domain of NRP1 [ xref , xref , xref ] at exposed Tyr297 and Asp320 residue sidechains [ xref , xref , xref ], primarily via an exon 8a-encoded arginine residue (CDKPRR; critical arginine underlined) with homologous interacting residues in tuftsin (TKPR) [ xref , xref ] and peptide ATWLPRR [ xref , xref ]."
"VEGF121 binding to the NRP1 b1 domain is mediated by the exon 8-encoded domain, which carries a C-terminal arginine residue ( xref , xref )."
"The finding that heparin was able to partially restore VEGF-A 165 binding to other NRP1 b1 domain mutants, which caused substantial loss of binding in the absence of heparin suggests, however, that heparin is able to mediate binding interactions between the VEGF-A 165 C terminus and the b1 domain."
"Tumor cell proliferation is induced by VEGF-A binding to NRP1 through a Ras-dependent mechanism. xref Adhesion to extracellular matrix and therefore motility and invasive capabilities are also affected by VEGF/NRP pathways."
"The library was tested for the ability to inhibit alkaline phosphatase (AP)-tagged VEGF-A binding to Nrp1 utilizing an in vitro plate-binding assay."
"Instead, it has been reported that NRP-1 binds VEGF-A to facilitate its interaction with VEGFR leading to more efficient stimulation of angiogenesis [ xref – xref ]."
"VEGF genes NRP1 and VEGFA interacted with APOE -ε4 on cognitive performance
(p.fdr<0.05)."
"NRP1 can bind to semaphorins instead of VEGFA and this impairs endothelial cell migration ( xref )."
"If NRP1 binds to semaphorin instead of VEGFA then endothelial cell migration is inhibited ( xref )."
"In NRP, the region comprising tandem coagulation factor V/VIII type domains b1 and b2 specifically interacts with the C-terminus of VEGF-A 165 with the b1 domain of NRP playing the most important role in VEGF-A binding xref , xref ."
"In this context, a human mAb (i.e., MNRP1685A) that blocks the binding of VEGF-A, VEGF-B, and PlGF to NRP-1 is currently under evaluation in clinical trials."
"Formation of the Nrp1/mutant GlyRS complex antagonises Nrp1 interaction with one of its main natural ligands, vascular endothelial growth factor-A (VEGF-A), contributing to neurodegeneration."
"In support of this idea, Zhang et al. provide evidence that VEGFR1 and NRP1 bind VEGF-A and prevent excessive VEGFR2 signaling that would otherwise promote button-to-zipper transformation and thereby reduce chylomicron uptake into lacteals."
"These results mean that down-modulation of phactr-1 expression is not solely due to the VEGF-A 165 binding to NRP-1 and/or VEGF-R1."
"Binding of VEGF-A to neuropilin 1 is essential for proangiogenic activity."
"VEGF-A binds to its receptors VEGFR1 (Flt-1), VEGFR2 (Flk-1), and coreceptors neuropilin 1 and 2."
"Nrp1 −/− hindbrains showed some defasciculation of these dorsolateral axons, but they were still clearly present in the mutant, suggesting that this are a suitable model to examine VEGFA isoform binding to NRP1."
"Here we report, novel promising small drug-like molecules disrupting the binding of VEGF-A₁₆₅ to NRP-1."
"Within the VEGF family, NRP1 binds VEGF-A, -B, -E and placenta growth factor (PlGF2),
whereas NRP2 binds VEGF-A, -C, -D, and PlGF2 ( xref ; xref )."
"It is of interest that we found fewer blood vessel capillaries in anti-Nrp-1B treated pups at day P5.5 (XREF_SUPPLEMENTARY), indicating that the formation of smaller blood vessels is impaired when VEGF-A binding to Nrp-1 is blocked, in line with previously reported effects of this antibody 24."
"To determine the effect of proteolytic activation of Sema3F on anti-angiogenic potency, we tested the ability of C-Sema to competitively block VEGF-A binding to neuropilin."
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"However, ITGAV rs3816375, NRP1 rs200809375, and VEGFA rs3024987 were not associated with PFS ( P >0.05)."
"The binding modes of both peptides were almost identical to those observed for Tuftsin and vascular endothelial growth factor A binding to the Nrp1 b1 domain."
"However, recent biochemical studies demonstrated that Sema3A and VEGF-A bind to distinct domains of NRP1 [ xref , xref ] and induce NRP1 endocytosis through different pathways, suggesting that these NRP1 ligands function separately, rather than competitively."
"While previous in-vitro studies suggested competitive binding of Nrp1 by VEGF-A and Sema3A ( xref ; xref ; xref ), more recent in-vivo data suggests that this may not be the case."
"NRP1 and VEGFA interacted with APOE -ε4 to modify
the association between ε4 and the final global cognition score."
"NRP1 and VEGFA interacted with APOE -ε4 on cross-sectional global cognition, so we
analyzed VEGFA and NRP1 isoform-specific
interactions with APOE -ε4 on this outcome, which showed
that pro-angiogenic coding transcripts of VEGFA ( VEGFA-207, VEGFA-205 ) and several protein coding
transcripts of NRP1 interacted with APOE -ε4 on global cognition ( xref )."
"In summary, we found that NRP1 and VEGFA interacted with APOE -ε4 on cognition."
"Experiments are performed both in the absence and presence of the soluble ligand vascular endothelial growth factor A (VEGFA), which binds to both VEGFR2 and NRP1."
"NRP1 is a coreceptor for the VEGF receptor (VEGFR), and VEGFA binding to the heterodimer of NRP1–VEGFR2 has enhanced VEGF signaling activity compared with binding of VEGFA to VEGFR1–VEGFR2."
"For both fibrosarcoma and
melanoma cell lines, they demonstrated the formation of trans NRP1-VEGFA-VEGFR2 complexes between endothelial cells and
NRP1-overexpressing tumor cells."
"Now, NRP1-VEGFA-VEGFR2 interactions in trans ( i.e. VEGFA bridging receptors across cells) are also coming into focus."
"These dimeric VEGF-A ligands then interact with VEGFR2 and NRP1 via separate ends of the peptide [ xref , xref ] ( xref )."
"Moreover, VEGF-A 165 b prevents the formation of neuropilin–VEGFR2 complexes by VEGF-A 165 a [ xref ]."
"VEGF-A 165 binding to VEGFR2 and neuropilin 1 induces a conformational change in VEGFR2 (REF. xref ), which is thought to be similar to that of the ERBB2 receptor xref , resulting in internal rotation of the intracellular domain."
"These results suggest that cooperative binding of VEGF-A to KDR and NRP-1 enhances KDR phosphorylation and its biological activities."
"VEGFA binding to both VEGFR2 and NRP1 on the same endothelial cell
forms a cis NRP1-VEGFA-VEGFR2 complex; the stoichiometry is likely two VEGFR2s, two NRP1s and one VEGFA."
"Co-immunoprecipitation analysis was employed to examine whether DDA affects VEGF-A-induced association of the NRP-1-VEGFR2 complex."