"In S. cerevisiae, ergosterol biosynthesis is repressed during osmotic and oxidative stress responses by the Hog1 dependent and -independent transcriptional downregulation of ERG11 and ERG2."
"Secondly, the erg24 gene disruptant and erg2erg24 double disruptant cells that are transformed by a hLBR expressing vector produce ergosterol and Delta 8 -sterols, respectively."
"ERG2 mediates the biosynthesis of ergosterol and is one of several genes that are involved in the biosynthesis of plasma membrane lipids that protect against acetic acid [XREF_BIBR]."
"These included simultaneous induction of all major EGR11 isoforms (CYP51A/B/C), over-expression of ERG2 and ERG6 to modulate ergosterol anabolism, and concurrent mobilization of Slt2-MAPK and CaMK signaling processes to overcome fungicide induced stresses."
"Although the overexpression of sterol C-8 isomerase encoded by ERG2 alone had negative effect on ergosterol biosynthesis, overexpression of ERG6 and ERG2 simultaneously led to an increased ergosterol level, which was 1.41-fold of that in empty vector strain, 1.92-fold of that in ERG2 only overexpressing strain and 1.12-fold of that in ERG6 only overexpressing strain."
"Erg2 was also inhibited by haloperidol, an observation consistent with the sigma receptor and erg2 being related gene products.There were also many genes whose expression varied by less than 2-fold."
"ERG2 was also inhibited by haloperidol, an observation consistent with the sigma receptor and ERG2 being related genes.Application of knockout approaches in mammalian cells is more challenging."
"~ re fused to lacZ, and transformed into the wild-type yeast strains, BWG1-7a and JRY527, and two ergosterol biosynthetic mutants, blocked a erg2 and hmgl."
"Direct interaction of primycin with the plasma membrane was demonstrated for the first time by using an ergosterol producing strain 33erg+ and its ergosterol less mutant erg-2."
"These included simultaneous induction of all major EGR11 isoforms (CYP51A/B/C), over-expression of ERG2 and ERG6 to modulate ergosterol anabolism, and concurrent mobilization of Slt2-MAPK and CaMK signaling processes to overcome fungicide induced stresses."
"ERG2 mediates the biosynthesis of ergosterol and is one of several genes that are involved in the biosynthesis of plasma membrane lipids that protect against acetic acid [XREF_BIBR]."
"Similar results were obtained by co-expressing hLBR and hSI, demonstrating that the two human enzymes can work together to complement the sterol biosynthesis defects induced by the erg2 and erg24 gene disruptions in the same cell."
"In S. cerevisiae, ergosterol biosynthesis is repressed during osmotic and oxidative stress responses by the Hog1 dependent and -independent transcriptional downregulation of ERG11 and ERG2."
"Although the overexpression of sterol C-8 isomerase encoded by ERG2 alone had negative effect on ergosterol biosynthesis, overexpression of ERG6 and ERG2 simultaneously led to an increased ergosterol level, which was 1.41-fold of that in empty vector strain, 1.92-fold of that in ERG2 only overexpressing strain and 1.12-fold of that in ERG6 only overexpressing strain."
"ERG2, ERG3, ERG10, DAN2, and DAN4 are activated by Upc2p solely in response to sterol depletion whereas DAN1 and TIR1 respond to both sterols and heme."
"Besides binding to the Zbtb16 promoter, Erg2 can bind to the Il2rb promoter, inducing the expression of CD122, a shared component of the IL2R and IL-15R."
"Besides binding to the Zbtb16 promoter ( xref ), Erg2 can bind to the Il2rb promoter ( xref ), inducing the expression of CD122, a shared component of the IL2R ( xref ) and IL-15R ( xref , xref )."
"ERG24, ERG2, and OPI1 deletions can be suppressed by the deletion (s) of SUR4 or FEN1 [XREF_BIBR, XREF_BIBR], encoding elongases, synthesizing very long chain 20-26-carbon FA that are components of another class of lipids, sphingolipids."
"IL-27 and T cell receptor signaling were found to promote the expression of the transcription factor Erg2, which was required for Lag3 expression and production of IL-10 by conventional CD4 + T cells."
"In addition, there was mutual regulated relationship between EGR2 and MIR-142-3P: ERG2 was encoded by the genes repressed by MIR-142-3P, while ERG2 associated with nerve growth factor-induced gene-A (NGFI-A) binding protein 2 binds to the pre-MIR-142-3p promoter to regulate its expression negatively ( xref )."
"In addition, there was mutual regulated relationship between EGR2 and MIR-142-3P : ERG2 was encoded by the genes repressed by MIR-142-3P, while ERG2 associated with nerve growth factor induced gene-A (NGFI-A) binding protein 2 binds to the pre-MIR-142-3p promoter to regulate its expression negatively."
"Synergy with geldanamycin was also observed between the allylamine terbinafine (FIC 90 = 0.25), which targets Erg1, and the morpholine fenpropimorph (FIC 90 = 0.016), which targets Erg24 and Erg2 (XREF_FIG)."
"In mice, cardia c -specific overexpression of the miR-499 transgene led to the development of cardiac hypertrophy and heart dysfunction by altering expression of contractile (MYH7B and skeletal muscle alpha-actin (ACTA1)) and conductivity (KCNH6 and K v 11.1) [196]."
"The signaling pathway was also investigated, and we found that low glucose can increase activation of s6k1 and ERG2 activation in GC cocultured T cells which was one of the signaling pathways regulating Th17 by mTOR.31 Taken together, we concluded that the increased percentage of Th17 might be as a result of increased miR-451 within the infiltrated T cells, and the increased miR-451 was GC exosome originated."
"We examined the effect of three other antifungal drugs : voriconazole, a triazole which also targets Erg11; fenpropimorph, which targets Erg2 and Erg24; and terbinafine, which inhibits squalene epoxidase."
"This study indicated that dyclonine modulates the yeast Erg2 protein, the yeast homolog of the mammalian sigma receptor that regulates potassium conductance."
"In addition to the monohydroxysterols found in the delta 8 goes to delta 7 isomerase blocked Saccharomyces cerevisiae mutant erg 2, a noval dihydroxysterol, ergosta-8,24 (38)-dien-3 beta,6 alpha-diol, was isolated."
"ERG2 binds to the Zbtb16 promoter region, which induces the expression of the TF promyelocytic leukemia zinc finger (PLZF) ( xref ), a master regulator of iNKT cell development and function ( xref )."
"TRH reduced the maximal available erg current amplitude by 12% (erg1), 13% (erg2) and 39% (erg3) and accelerated the time course of erg1 and erg2 channel deactivation, whereas erg3 deactivation kinetics were not significantly altered."
"Despite the high sequence homology between the sigma-1 receptor and yeast sterol isomerase, overexpression of the sigma-1 receptor failed to rescue an ERG2 deficient strain of the yeast Saccharomyces cerevisiae [XREF_BIBR]."
"For example it was found that deletion of ISC1 increased a erg3Delta erg6Delta double mutant temperature tolerance defect, as well as the erg3Delta and erg4Delta sorbic acid sensitivity phenotypes, and deletion of SUR2 or SCS7 suppressed slow growth of erg2 a low temperature."
"ETS-1 protein is phosphorylated by Ca (2+)-dependent events, whereas ERG-2 protein is phosphorylated by activation of protein kinase C, suggesting their involvement in distinct signal transduction mechanisms."
"Transcriptional analyses show that Mot3 represses transcription of ERG2, ERG6 and ERG9, genes required for ergosterol biosynthesis, during both aerobic and hypoxic growth."
"Lovastatin treatment enhanced the expression of ergosterol biosynthetic genes, ERG2 and ERG3 in wild-type and upc2BDelta but not in upc2ADelta cells."
"The microarray expression profiles showed that genes upregulated in ACC1 overexpressing strains are FAS1, ERG11, ERG28, ERG5, ERG2 and ERG20, supporting the observed increase of zymosterol and saturated fatty acids."
"Pheromone induced P FUS1 -lacZ transcription was attenuated by the erg2, 3, and 6 and lcb1 ts mutations and also by inhibiting ergosterol synthesis with FLZ or inhibiting sphingolipid synthesis with myriocin."
"In addition, there was mutual regulated relationship between EGR2 and MIR-142-3P: ERG2 was encoded by the genes repressed by MIR-142-3P, while ERG2 associated with nerve growth factor-induced gene-A (NGFI-A) binding protein 2 binds to the pre-MIR-142-3p promoter to regulate its expression negatively ( xref )."
"In addition, there was mutual regulated relationship between EGR2 and MIR-142-3P : ERG2 was encoded by the genes repressed by MIR-142-3P, while ERG2 associated with nerve growth factor induced gene-A (NGFI-A) binding protein 2 binds to the pre-MIR-142-3p promoter to regulate its expression negatively."
"Consistent with our findings that ERG5 is an HSP90 genetic interactor, previous analyses of C. albicans strains resistant to the membrane disrupting antifungal drug amphotericin B demonstrated that deletion of the ergosterol biosynthesis genes ERG6, ERG2, or combined deletion of both ERG3 and ERG11 caused hypersensitivity to geldanamycin."
"The delta 8 --> delta 7 sterol isomerase produced by the M. grisea ERG2 gene exhibited a level of sensitivity to the sterol biosynthesis inhibitor, tridemorph, similar to that of the enzyme derived from the U. maydis ERG2 gene."
"ERG2 binds to the Zbtb16 promoter region, which induces the expression of the TF promyelocytic leukemia zinc finger (PLZF) ( xref ), a master regulator of iNKT cell development and function ( xref )."
"Here, it is shown that the deletion in ERG2, which encodes sterol C8-C7 isomerase (the next enzymatic step after Erg6), promotes the vacuolar degradation of Tat2."
"In addition, a 2u plasmid overexpression of the ERG2 sequence did not produce Fp ^ Characterization studies were focused on plasmid pMLIOO, because it was the only plasmid to confer FpR consistently when tested in a number of different genetic backgrounds."
"Pheromone induced P FUS1 -lacZ transcription was attenuated by the erg2, 3, and 6 and lcb1 ts mutations and also by inhibiting ergosterol synthesis with FLZ or inhibiting sphingolipid synthesis with myriocin."
"Deletion of erg2 also induced the expression of erg24, the gene encoding C-14 sterol reductase, but not other tested erg genes which were responsive to erg11 inactivation."
"Besides binding to the Zbtb16 promoter ( xref ), Erg2 can bind to the Il2rb promoter ( xref ), inducing the expression of CD122, a shared component of the IL2R ( xref ) and IL-15R ( xref , xref )."
"IL-27 and T cell receptor signaling were found to promote the expression of the transcription factor Erg2, which was required for Lag3 expression and production of IL-10 by conventional CD4 + T cells."
"In S. cerevisiae, ergosterol biosynthesis is repressed during osmotic and oxidative stress responses by the Hog1 dependent and -independent transcriptional downregulation of ERG11 and ERG2."
"Moreover, our transcriptome study implies that a pulsatile GnRH application downregulates the expression of Kcna2 (K v 1.2) and Kcnh6 (ether-a-go-go), while it upregulates Kcnk10 (outward rectifier) and Na + / Ca 2+ exchanger Slc24a3, indicating that GnRHR may indirectly be involved in regulation of cell excitability."
"ERG24, ERG2, and OPI1 deletions can be suppressed by the deletion (s) of SUR4 or FEN1 [XREF_BIBR, XREF_BIBR], encoding elongases, synthesizing very long chain 20-26-carbon FA that are components of another class of lipids, sphingolipids."
"These enzymes are indicated in Fig. 2 and are : (i) from XI to X, a AS-AZ-sterol isomerase (product of ERG2); and (ii) from X to XII a AT-sterol-5-desaturase (product of ERG3), a A22-desa- turase (product of ERG5) and a A24-reductase (product of ERG4) [15,25]."
"For example it was found that deletion of ISC1 increased a erg3Delta erg6Delta double mutant temperature tolerance defect, as well as the erg3Delta and erg4Delta sorbic acid sensitivity phenotypes, and deletion of SUR2 or SCS7 suppressed slow growth of erg2 a low temperature."
"This was further supported by the fact that when the erg2 mutant was treated with ketoconazole, upstream inhibition overrode the effects by downstream inhibition on ergosterol biosynthesis pathway."